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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal <t>nerve</t> <t>stimulation</t> (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures <t>ANOVA,</t> Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.
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Effects of tibial nerve (A, B) and spinal nerve stimulation (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures ANOVA, Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.

Journal: Neurourology and Urodynamics

Article Title: Differentiation and interaction of tibial versus spinal nerve stimulation for micturition control in the rat

doi: 10.1002/nau.22506

Figure Lengend Snippet: Effects of tibial nerve (A, B) and spinal nerve stimulation (C, D: 10 Hz, pulse width 0.1 msec) on the frequency of the bladder rhythmic contraction. A, C: Time course response of frequency of the bladder rhythmic contraction to bilateral tibial nerve (A) and spinal nerve (C) stimulation at motor threshold (T mot ) and threefold of T mot (3 times T mot ). Shaded areas are responses during electrical stimulation. +, P < 0.05, versus control without stimulation; repeated measures ANOVA, Bonferroni post-test. B, D: Intensity dependent effects of unilateral (uni) and bilateral (bi) tibial nerve (B) and spinal nerve (D) stimulations on frequency of bladder contractions during electrical stimulation. X -axis denoted increasing current intensity relative to multiples of motor threshold (T mot ) stimulation. The mean contraction frequency during stimulation is expressed as a percentage of the control response prior to stimulation (% control). *, P < 0.05, versus values without stimulation, unpaired Student's t -test; #, P < 0.05, first 5-min stimulation versus second and third 5-min stimulation, *, P < 0.05, unilateral versus bilateral, unpaired Student's t -test. The number of animals is indicated in each symbol.

Article Snippet: Time course for the BRC response to stimulation was analyzed using repeated measures ANOVA (Prism 5, GraphPadSoftware, Inc., San Diego, CA).

Techniques: Control